Figure 5

TUNEL staining. A: The TUNEL-positive material was localized in the nuclei of neurons as shown in the MCAO group. The damaged cells were characterized by a round and shrunken morphology. Fewer TUNEL positive cells were observed in hydrogen treated animals. B: Hydrogen saline applied 3 h after reperfusion markedly reduced the number of TUNEL positive cells when compared with normal saline treated group (*P< 0.05 vs MCAO). (a, b, c, d, e, f, g, h, i: 100 ×; j, k, l, m, n, o, p, q, r: 200 ×; Scale bar: 20 μm).