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Figure 2 | Medical Gas Research

Figure 2

From: Molecular hydrogen protects chondrocytes from oxidative stress and indirectly alters gene expressions through reducing peroxynitrite derived from nitric oxide

Figure 2

Hydrogen protects chondrocytes of fibrocartilages from cell death. (A) Meniscus fibrocartilages from SD rats was incubated with 1 mM SNAP in the presence or absence of hydrogen for 0, 6, 20, 48, or 80 hr at 37°C. Cells were stained with calcein AM (Live cell: green) and ethidium homodimer (Dead cell: red) as described in Materials and methods. Scale bar: 40 μm. (B) Chondrocyte viability was determined by counting green and red cells from three regions of each slice. Six slices were used for each experimental group. The slices were incubated with 1 mM SNAP in the presence or absence of hydrogen for the indicated periods at 37°C. Data are the means ± SD (n= 6). *p< 0.05; ***p< 0.001. (C) The slices were incubated with 0, 0.3, 1, or 3 mM SNAP in the presence or absence of hydrogen for 48 hr at 37°C. Data are the means ± SD (n= 6). *p< 0.05; ***p< 0.001.

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