Figure 3

Hydrogen decreases nitrotyrosine in chondrocytes and cartilage matrix. (A) Meniscus fibrocartilage from SD rats was incubated with 1 mM SNAP in the presence or absence of hydrogen for 3 hr at 37°C. Frozen sections were stained with anti-nitorotyrosine antibody and visualized with DAB as described in Methods. The inset shows chondrocytes. Cartilage incubated with 1 mM SIN-1 and without SNAP was used for positive and negative staining controls, respectively. Scale bar: 40 μm. (B) Levels of nitrotyrosine in cartilage were estimated from anti-nitrotyrosine immunostaining using an image analysis program, Image J program. Data are the mean ± SD (n = 4). *p < 0.05; control versus hydrogen in 1 mM SNAP-treated groups.