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Figure 4 | Medical Gas Research

Figure 4

From: Molecular hydrogen protects chondrocytes from oxidative stress and indirectly alters gene expressions through reducing peroxynitrite derived from nitric oxide

Figure 4

Hydrogen alters mRNA and protein expressions of matrix proteins and matrix-metalloproteases (MMPs). Meniscus fibrocartilage from rats was incubated with 1 mM SNAP in the presence or absence of hydrogen for 4 hr or 20 hr at 37°C. Total RNA was extracted from 4 hr-incubated cartilage and the expression levels of aggrecan (A), type II collagen (B), GAPDH (C), MMP3 (D) and MMP13 (E) were analyzed by real-time PCR coupled with reverse transcription. Data are the mean ± SD (n = 4). *p< 0.05; **p< 0.01. (F) Total protein was extracted from 20 hr-incubated cartilage and the expression levels of aggrecan, MMP13 and actin were analyzed by immunoblotting.

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Medical Gas Research

ISSN: 2045-9912

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