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Figure 3 | Medical Gas Research

Figure 3

From: Moderately delayed post-insult treatment with normobaric hyperoxia reduces excitotoxin-induced neuronal degeneration but increases ischemia-induced brain damage

Figure 3

Effects of NBO on NMDA-evoked Ca2+influx in cortical neuronal cultures and OGD-induced LDH release in acute brain slices. (A)Exposure to NMDA (25 μM) produces a rapid increase in intracellular free Ca2+, which recovered over the following minutes. NBO reduced NMDA-induced Ca2+influx by ~20%. For each experiment, N = 3, n = 90-120. (B)Exposure to OGD produces an increase in LDH release, as a marker of cell injury. NBO increases OGD-induced LDH release by ~25% as compared with OGD control slices treated with medical air. n = 23-26 per condition. Data are expressed as mean ± SEM. #P< 0.0005 vssham; * P <0.001 vsNMDA or OGD.

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