Figure 4

Effects of ACS6 on the release of Cyt-c in PC12 cells. After pretreated with 16 μmol/L ACS6 for 30 min, PC12 cells were exposed to 5 mmol/L homocysteine (Hcy) for 24 h. A, representative immunoblots for Cyt-c release from three independent experiments. Cytosolic fractions of the extract were subjected to Western blot analysis using an anti-Cyt-c antibody. In all blots, staining for β-actin was used as a loading control. B, quantification of cytosolic Cyt-c accumulation as a percent of the control. Values are the mean ± SEM (n= 3). ***P <0.001, versus control; ^#P< 0.05, versus 5 mmol/L homocysteine-treated alone group.