Figure 8

Effects of ACS6 on the expression of Bcl-2 in PC12 cells. After pretreated with 16 μmol/L ACS6 for 30 min, PC12 cells were exposed to 5 mmol/L homocysteine (Hcy) for 24 h. The levels of Bcl-2 expression in PC12 cells were determined by Western blot using an anti-Bcl-2 antibody. Western blot images show representative results from three independent experiments. In all blots, staining for β-actin was used as a loading control. The level of Bcl-2 expression obtained in each experimental condition was calculated as a fold of the control. Values are the mean ± SEM (n= 3). **P <0.01, versus control; ^#P< 0.05, versus 5 mmol/L homocysteine-treated alone group.