Figure 1
NBO treatment inhibits gp91phoxinduction in ischemic brain tissue after 90-min MCAO with 22.5 hrs of reperfusion. Nonischemic (Non-I) and ischemic (I) hemispheric brain tissue was homogenized for analyzing gp91phoxprotein levels with western blot. As a loading control, the blots were stripped and reprobed with β-actin. A)Representative blots of gp91phoxand corresponding β-actin. B)The relative quantity of protein was calculated after normalization to β-actin. Gp91phoxexpression was markedly increased in the ischemic brain tissue, which was significantly inhibited by NBO. *P< 0.05 versus normoxic (NA) Non-I group, n = 6; #p < 0.05 versus normoxic (NA) I group, n = 6.