Figure 5

NBO treatment or gp91^phoxknock-out significantly reduces occludin degradation in ischemic brain tissue after 90-min MCAO with 22.5 hrs of reperfusion. Hemispheric brain tissue was homogenized for analyzing tight junction proteins occludin and claudin-5 with western blot. A)Representative blots of occludin, claudin-5 and the corresponding β-actin showing their changes in Nonischemic (Non-I) and ischemic (I) brain tissues in normoxic wild-type (NA-WT), NBO-treated WT (NBO-WT), normoxic gp91^phoxknock-out (NA-KO) and NBO-KO mice. B)The relative quantity of occludin protein was calculated after normalization to β-actin. Occludin protein level was significantly reduced in the ischemic brain of NA-WT mice, and this reduction was significantly reversed by NBO or gp91^phoxKO. *p < 0.05 versus Non-I, and ^#p < 0.05 versus NA-WT, n = 6. C)No significant changes were observed in claudin-5 protein for all groups.