Effect of HRW administration on glomerular sclerosis. After 16 weeks of HRW administration, coronal sections of the renal tissue from distilled water-administered rats (control, n = 8) and HRW-administered rats (HRW, n = 7) were stained with PAS and examined by light microscopy in a blinded fashion (Figure 3A). Glomerulosclerosis was semi-quantitatively evaluated according to the criteria developed by Uehara et al. . *P < 0.05. Data represent mean ± SE. Figure 3B illustrates the relationship between the glomerulosclerosis score and concentrations of blood urea nitrogen. White and black circles indicate control and HRW rats, respectively.