Figure 1
From: Isoflurane does not cause neuroapoptosis but reduces astroglial processes in young adult mice
Immunohistochemical staining of apoptotic and anti-apoptotic signals in the hippocampal Cornu Ammonis layer 1 (CA1) regions. A-C: Activated caspase-3 staining (green) revealed no apoptosis in isoflurane-exposed animals (A), whereas positive control from a mouse after 20-min of hypoxia exhibited activated caspase-3+cells (B). C is higher magnification of area outlined in B. D-F: Bcl-xl immunostaining in the hippocampal CA1 regions (red) revealed no anti-apoptotic response in isoflurane-exposed animals (D), whereas positive control from a mouse after 20 min of hypoxia exhibited Bcl-xl+cells (E). F is a higher magnification of area outlined in E. Cell nuclei are stained with DAPI (blue).