Figure 5
From: Carbon monoxide inhibits Fas activating antibody-induced apoptosis in endothelial cells
CO activated ERK and NF-κB signaling. MLEC cultures were pretreated in the absence or presence of CO (250 ppm) for 2 h prior to the addition of antibody Jo2 (200 ng/ml) for the indicated times. The total lysates were subjected to Western blot to detect ERK or NF-κB p65 phosphorylation. Total p65 served as the standard. Westerns are representative of three independent experiments.