Figure 3

Effect of LPS and hydrogen sulfide inhalation on lung inflammation. As controls, mice received phosphate buffered saline (PBS, intranasally) and were kept in room air or in 80 ppm H₂S for 6 h (plus 1 h pretreatment). LPS-treated mice (LPS treatment, i.n.) were either kept in room air or in 80 ppm H₂S for 6 h (plus 1 h pretreatment). Bronchoalveolar lavage was performed in the right lung. The relative amount of neutrophils (A) was determined by cytospin analysis, and the amount of IL-1β (B) and MIP-2 (C) was determined by ELISA. Graphs represent means ± SEM, n = 8/group. ANOVA on Ranks (Dunn`s posthoc test), *P < 0.05 vs. PBS + air group; ^#P < 0.05 vs. PBS + H₂S group; ^§P < 0.05 vs. LPS + H₂S group.