Figure 3

CO application diminished the levels of IFN-γ⁺, IL-17⁺but not TNF-α⁺cells. The percentage of TNF-α⁺cells in decidua (a), spleen (b) and iliac lymph nodes (c) of pregnant mice was not modified by treatment with 50 ppmCO. The percentage of IFN-γ⁺cells in decidua (d) and spleen (e) were significantly decreased after 50 ppmCO treatment as compared to animals which received mixed air. No differences were found for the levels of these molecules in lymph nodes (f). CO treatment lead to a significant diminution in the percentage of IL17⁺cells in decidua, spleen and lymph nodes (g, h, i). Pregnant animals were treated either with mixed air or 50 ppmCO during days (3-8 of pregnancy) and sacrificed on day 8 of pregnancy. Percentage of cells positive for TNF-α⁺, IFN-γ⁺or IL-17⁺were analyzed by flow cytometry and expressed as dot plots showing the median. Statistical significances were analyzed by the non-parametric Mann-Whitney U-test between two groups. *:p < 0.05 and **: p < 0.01.