Amelioration of cardio-renal injury with aging in dahl salt-sensitive rats by H2-enriched electrolyzed water

Medical Gas Research

Table 4 Comparison of oxidant and anti-oxidant systems between FW and EW groups at 48 wks

	FW	EW
Kidney
Nitrotyrosine (positive area%)	6.3 ± 0.7	4.3 ± 0.3^a
Malondialdehyde (positive area%)	60.5 ± 1.2	57.2 ± 1.2^a
ED1	113.5 ± 13.4	75.3 ± 8.2^a
Heart
Nrf2 (Nrf2/beta actin)	1.0 ± 0.4	2.4 ± 0.6^a
Number of Nrf2 (per slide)	1.9 ± 0.4	10.7 ± 1.4^a
gp91phox (gp91phox/beta actin)	7.5 ± 2.4	1.1 ± 0.4^a
Nitrotyrosine (positive area%)	49.8 ± 6.0	37.8 ± 6.7^a
Malondialdehyde (positive area%)	63.7 ± 2.6	44.6 ± 1.2

(mean±SEM).
FW filtered water, EW water with dissolved H₂ produced by electrolysis, DW dehydrogenated EW.
Nrf2, nuclear factor (erythroid-derived 2)-like 2.
NADPH oxidase activity, nicotinamide adenine dinucleotide phosphate-oxidase.
gp91phox and p40phox, phagocytic oxidase unit of NADPH oxidase.
SOD2, Superoxide dismutase 2.
^a P < 0.05, EW vs. FW.

ISSN: 2045-9912