Effects of hydrogen on fatty acid uptake and lipid accumulation in HepG2 cells. (A) After treatment with or without hydrogen for 24 h, cells were exposed to 0.3 mM palmitate-BSA (PA) for 24 h, and then stained with Oil Red O. The Oil Red O content was measured at a wavelength of 540 nm. Data are expressed as mean ± SD (n = 6) (***p < 0.001). (B) After treatment with or without hydrogen for 24 h, cells were exposed to BODIPY-FL C16 for 15 min and fluorescence intensity was measured (*p < 0.05).