Figure 3

Effects of hydrogen on JNK phosphorylation and lipid raft integrity in HepG2 cells. (A) After treatment with or without hydrogen for 24 h, cells were incubated in the presence of palmitate-BSA (0.3 mM) for indicated time periods, and cell lysates were harvested and subjected to Western blot analysis for JNK and phospho-JNK proteins. (B) After treatment with or without hydrogen for 24 h, HepG2 cells were exposed to Alexa594-CT-B for 1 h at 37°C and fluorescent intensity was measured with the fluorescence microplate reader.