Figure 4

Effects of hydrogen on CD36 expression in HepG2 cells. After treatment with or without hydrogen for 24 h, total RNA was harvested and quantitative real-time RT-PCR was performed for CD36 mRNA (A), and cell lysates were harvested and Western blot analysis was performed for CD36 protein (B). GAPDH was used as an internal control, and the CD36 protein level (fold) was evaluated by densitometry (*p < 0.05).