Expression analysis following argon treatment in an in vivomodel of transient middle cerebral artery occlusion in rats

Fahlenkamp, Astrid V; Coburn, Mark; de Prada, Antonio; Gereitzig, Nadine; Beyer, Cordian; Haase, Hajo; Rossaint, Rolf; Gempt, Jens; Ryang, Yu-Mi

doi:10.1186/2045-9912-4-11

Table 1 Primer sequence, product length and characteristics

From: Expression analysis following argon treatment in an in vivomodel of transient middle cerebral artery occlusion in rats

Transcript	Sequence	Orientation	Annealing temperature (°C)	Transcript size (base pairs)	Melting point (°C)	GeneBank_ID
Cyclophilin	GGCAAATGCTGGACCAAACAC	Sense	60	196	82	NM_017101.1
	TTAGAGTTGTCCACAGTCGGAGATG	Antisense			82	NM_017101.1
Tumor nekrose faktor alpha	CTCCCAGAAAAGCAAGCAACC	Sense	57	210	85	NM_012675.3
(TNFα)	CGAGCAGGAATGAGAAGAGG	Antisense			85	NM_012675.3
Interleukin-1β	CTGTGACTCGTGGGATGATG	Sense	60	210	81	NM_031512.2
(IL-1β)	GGGATTTTGTCGTTGCTTGT	Antisense			81	NM_031512.2
Interleukin-6	CCGGAGAGGAGACCTCACAG	Sense	60	161	78	NM_012589.1
(IL-6)	ACAGTGCATCATCGCTGTTC	Antisense			78	NM_012589.1
Chemokine (C-C) ligand 2	CCAGAAACCAGCCAACTCTC	Sense	59	192	83	NM_031530.1
CCL2	CCGACTCATTGGGATCATCT	Antisense			83	NM_031530.1
Matrix metalloproteinase-9	CCACCGAGCTATCCACTCAT	Sense	60	180	79	NM_031055.1
MMP-9	GTCCGGTTTCAGCATGTTTT	Antisense			79	NM_031055.1
Hypoxia inducible factor 1	TCAAGTCAGCAACGTGGAAG	Sense	60	198	81	NM_024359.1
alpha (HIF-1α)	TATCGAGGCTGTGTCGACTG	Antisense			81	NM_024359.1
Chemokine (C-C) ligand 5	TGCCCACGTGAAGGAGTATTTTTA	Sense	60	81	78	NM_031116.3
(CCL5)	TGGCGGTTCCTTCGAGTGACAA	Antisense			78	NM_031116.3
Transforming growth factor β (TGF-β)	GGGACTCTCCACCTGCAAGAC	Sense	63	393	88	NM_012578.2
Transforming growth factor β (TGF-β)	CTCTGCAGGCGCAGCTCTG	Antisense			88	NM_012578.2
Nerve growth factor	GAAACGGAGACTCCGTTCACC	Sense	60	100	82	NM_001067130.1
(NGF)	GATTGTACCATGGGCCTGGA	Antisense			82	NM_001067130.1
Vascular endothelial growth factor α (VEGFα)	CCTGGTGGACATCTTCCAGGAGTACC	Sense	60	196	84	NM_031836.2
Vascular endothelial growth factor α (VEGFα)	GAAGCTCATCTCTCCTATGTGCT	Antisense			84	NM_031836.2
Inducible NO-synthetase	CACCTTGGAGTTCACCCAGT	Sense	59	179	85	NM_012611.3
(iNOS)	ACCACTCGTACTTGGGATGC	Antisense			85	NM_012611.3
CD3	AGAACTGCATGGAGGTGGAC	Sense	60	232	84	NM_001108140.1
	TTTCGGATGGGCTCATAGTC	Antisense			84	NM_001108140.1
CD11b	TTACCGGACTGTGTGGACAA	Sense	62	219	82	NM_012711.1
	AGTCTCCCACCACCAAAGTG	Antisense			82	NM_012711.1
2’3’-Cyclic nucleotide 3’ phosphodiesterase (CNP)	TGGCGAAGAAGATGGAAGTCA	Sense	60	257	82	M18630.1
2’3’-Cyclic nucleotide 3’ phosphodiesterase (CNP)	GTGGGTGAAGGAACTGATGGTT	Antisense			82	M18630.1
Glial fibrillary acid protein	AGAAAACCGCATCACCATT	Sense	60	188	83	NM_017009.2
(GFAP)	GCACACCTCACATCACATCC	Antisense			83	NM_017009.2
Insulin-like growth factor 1	TGATCTGAGGAGGCTGGAGATGTA	Sense	59	95	82	NM_178866.4
IGF-1	CTTCTGAGTCTTGGGCATGTCA	Antisense			82	NM_178866.4

A full summary of the primer sequences employed for gene expression analysis plus their characteristics is given in Table 1.

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ISSN: 2045-9912

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