Figure 2

Multiple sevoflurane anesthesia in P6 mice decreases levels of P-GSK3β(ser9) and P-AKT(ser473) in brain tissues. A. Anesthesia with 3% sevoflurane two hours daily for three days in P6 mice decreases the levels of P-GSK3β(ser9) in the brain tissues of the mice as compared to the control condition. There is no significant difference in β-Actin levels in the brain tissues of the mice between the sevoflurane anesthesia and control condition. B. Quantification of the Western blot shows that the sevoflurane anesthesia decreases the levels of P-GSK3β(ser9) in the brain tissues of the mice as compared to the control condition. C. Anesthesia with 3% sevoflurane two hours daily for three days in P6 mice decreases the levels of P-AKT(ser473) in the brain tissues of the mice as compared to the control condition. There is no significant difference in β-Actin levels in the brain tissues of the mice between the sevoflurane anesthesia and control condition. D. Quantification of the Western blot shows that the sevoflurane anesthesia decreases the levels of P-AKT(ser473) in the brain tissues of the mice as compared to the control condition. P, phosphorylated; GSK3β, glycogen synthase kinase 3β. N = 6.