Figure 3

Short sevoflurane treatment increases the levels of P-GSK3β(ser9) and P-AKT(ser473) in H4 cells. A. Anesthesia with 4% sevoflurane for two hours increases the levels of P-GSK3β(ser9) in the H4 cells as compared to the control condition. There is no significant difference in β-Actin levels in the H4 cells between the sevoflurane anesthesia and control condition. B. Quantification of the Western blot shows that the sevoflurane anesthesia increases the levels of P-GSK3β(ser9) in the H4 cells as compared to the control condition. C. Anesthesia with 4% sevoflurane for two hours in H4 cells increases the levels of P-AKT(ser473) in the H4 cells as compared to the control condition. There is no significant difference in β-Actin levels in the H4 cells between the sevoflurane anesthesia and control condition. D. Quantification of the Western blot shows that the sevoflurane anesthesia increases the levels of P-AKT(ser473) in the H4 cells as compared to the control condition. P, phosphorylated; GSK3β, glycogen synthase kinase 3β. N = 6.