Increase of mitochondrial membrane potential in sperm suspensions by H
treatment. Six sperm suspensions were either untreated (a), or treated with H2- or N2-mixed gases (b and c, respectively), and stained with MitoTracker Green (MTG) and TMRM mitochondria- and mitochondrial membrane potential-sensitive fluorescent dyes. Differential interference contrast (DIC) images are also shown. Bars = 10 μm. The fluorescence intensity of each TMRM-stained sperm was semi-quantitatively analyzed by ImageJ (d). Data represent means ± SD. ***P < 0.001, versus the untreated sperm suspension.