Figure 1

Flow-chart of experiment. All animals (n = 28) were submitted to intramuscular anesthisia (Ketamine 50 mg/kg and Xylazin 10 mg/kg), then divided to four groups: Saline rats (n = 7): submitted to intracolonic infusion of saline solution, no chamber pressurization; Saline/HBO rats: submitted to intracolonic infusion of saline solution plus two sessions of HBO (n = 7); TNBS rats, submitted to intracolonic infusion of TNBS and no chamber pressurization (n = 7); TNBS/HBO rats, submitted to intracolonic infusion of TNBS plus two sessions of HBO (n = 7). After the second HBO session all animals were submitted to intramuscular anesthesia, laparotomy, colon extraction, macroscopic damage score and the colon was divided into four segments, of 1 cm each (First segment: MPO; Second: Cytokines; Third: Western Blot (COX-2 and iNOS); Fourth: Immunohistochemistry (COX-2 and HIF-1α).