Figure 4
From: Hyperbaric oxygen therapy ameliorates TNBS-induced acute distal colitis in rats
Hyperbaric oxygen reduces tissue COX-2 and iNOS expression. (A) Acute distal colitis was induced by the intracolonic injection of TNBS (150 mg/kg). After two sessions of hyperbaric oxygen, the rats were killed, the distal 6 cm colonic segment was removed, and the expression of COX-2 was determined by western blot. The β-Actin level was used as a control. Data are presented as representative blots. (B) Densitometry of the pixel intensity of COX-2 bands relative to β-Actin is present. (C) Expression of iNOS was determined by western blot. The b-actin level was used as a control. (D) Densitometry of the pixel intensity of iNOS bands relative to β-Actin is present. Data are presented as representative blots. Data are expressed as the mean ± S.E.M. (n = 5–7). # P < 0.01 compared to the saline-injected group; * P < 0.01 compared to colitis (TNBS) group (one-way ANOVA followed by the Bonferroni’s test).