Figure 4

8-OHdG staining. A: No 8-OHdG positive staining was obtained in sham animals. Marked 8-OHdG positive staining was observed in the normal saline treated animals and the staining was localized to the nuclei of neurons. Hydrogen saline animals showed fewer number of 8-OHdG positive cells. B: Hydrogen saline applied at 3 h after reperfusion significantly reduced 8-OHdG positive cells when compared with MCAO group (**P < 0.01 vs. MCAO). (a, b, c, d, e, f, g, h, i: 100 ×; j, k, l, m, n, o, p, q, r: 200 ×; Scale bar: 20 μm).