Effect of an hyperbaric nitrogen narcotic ambience on arginine and citrulline levels, the precursor and co-product of nitric oxide, in rat striatum

Table 1 Comparison of the striatal citrulline baseline with its evolution in nitrogen at 3 MPa (n = 6), in helium at 3MPa (n = 8), at atmospheric pressure with NMDA (2 mM) retrodialysis in the striatum (n = 8), or in nitrogen exposure with NMDA retrodialysis in the striatum (n = 6), at atmospheric pressure with KCL (100 mM) and Saclofen (1 mM) retrodialysis in the PFC (n = 9), or in nitrogen exposure with KCL and Saclofen retrodialysis in the PFC (n = 9).

Citrulline	Control groups		3MPa Nitrogen groups		Comparison
	Comp.	180 Minutes exposure Median (1^st_ 3^rdquartile)	Comp.	180 Minutes exposure Median (1^st_ 3^rdquartile)	p-value
	p-value	p-value post hoc test	p-value	p-value post hoc test	comp	Stay
Helium at 3MPa(1)/Nitrogen(2) n = 8/6	*0.002*	112.4 (91.3_151.7) 0.220	0.544	82.8(67.5_97.7) *0.005*	*0.008*	*< 0.001*
NMDA retrodialysis(3_4) n = 8/6	0.079	168.5(142.7_216.3) *< 0.001*	0.720	94.3(58.7_169.1) 0.327	0.939	*< 0.001*
PFC retrodialysis(5_6) n = 9/9	*0.009*	119.0(100.0_150.8) *0.012*	0.141	135.7(84.1_167.1) *0.007*	0.397	0.727

-1- Baselines and periods at maximum pressure (but not the compression stage) were described using median and quartile. -2- The citrulline baseline was compared to the compression stage (up to 3MPa-"Comp.") and to a maximum pressure period (at 3MPa, "180 Minutes exposure") using a Kruskal-Wallis test (p-values were noted, α = 5%), followed by a post-hoc test transcribed by squares. Each square represents a string of samples (~median value) that must be compared with the baseline samples, arranged in chronological order: 2 squares for 2 series of samples available during the compression stage (or simili); 9 squares for 9 series of samples available during the period at the maximum pressure (or simili). Dark squares indicate a significant difference, as indicated by a post-hoc Dunn test (α = 5%), between samples at the corresponding time point and its baseline. White squares indicate no significant difference between the string of samples at the corresponding time point and the baseline. The compression stages (up to 3MPa) and the maximum pressure period (3MPa) of the control groups and the 3MPa nitrogen group were compared with each other, using a Kruskal-Wallis test (α = 5%). P-values were noted.

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