Table 1 Primers and probes for RT-PCR.
Gene | Â | Sequence |
|---|---|---|
aggrecan | F primer | 5'-GACCAGGAGCAATGTGAGGAG-3' |
| Â | R primer | 5'-CTCGCGGTCGGGAAAGT-3' |
| Â | probe | 5'-CCAAGTTCCAGGGCCACTGTTATCGC-3' |
type II collagen | F primer | 5'-TTGGAGAGACCATGAACGGC-3' |
| Â | R primer | 5'-TTAGCGGTGTTGGGAGCC-3' |
| Â | probe | 5'-CACTTCAGCTACGGCGACGGCAA-3' |
MMP3 | F primer | 5'-TCCCAGGAAAATAGCTGAGAACTT-3' |
| Â | R primer | 5'-AAACCCAAATGCTTCAAAGACAG-3' |
| Â | probe | 5'-CCAGGCATTGGCACAAAGGTGGA-3' |
MMP13 | F primer | 5'-TGGAGTTATGATGATGCTAACCAGAC-3' |
| Â | R primer | 5'-TGTCGCCAATTCCAGGGA-3' |
| Â | probe | 5'-TGGACAAAGACTATCCCCGCCTCATAGAAG-3' |
GAPDH | F primer | 5'-CATCACTGCCACCCAGAAGA-3' |
| Â | R primer | 5'-ATGTTCTGGGCAGCC-3' |
| Â | probe | 5'-TGGATGGCCCCTCTGGAAAGCTG-3' |