Effects of ACS6 on homocysteine-exerted accumulation of intracellular ROS in PC12 cells. After pretreated with 16 μmol/L ACS6 for 30 min, PC12 cells were exposed to 5 mmol/L homocysteine (Hcy) for 24 h and stained with DCFH-DA for 20 min. The changes of ROS in different treatment groups were quantified by fluorescent sorting FCM analysis (A, B). (A) Representative histogram of DCF-derived fluorescence in PC12 cells exposed to different treatments measured by FCM. (B) Quantitative analysis of the mean fluorescence intensity (MFI) of DCF measured by FCM. Values are the mean ± SEM (n= 3). ***P <0.001, versus control; ###P< 0.001, versus 5 mmol/L homocysteine- treated alone group.