Figure 3

Hyperbaric oxygen reduces tissue myeloperoxidase activity (MPO) and pro-inflammatory cytokine expression in acute distal colitis. (A) Acute distal colitis was induced by an intracolonic injection of TNBS (150 mg/kg). After two sessions of hyperbaric oxygen (HBO) the rats were killed, the distal 6 cm colonic segment was removed, and the MPO activity was measured. The anti-inflammatory effect of HBO was detected as a decrease in the number of tissue neutrophils. (B) Colitis by TNBS increases the IL-1β tissue expression and HBO decreases its expression. (C) Colitis by TNBS increases the TNF-α tissue expression. There was not a significant decrease in expression after HBO. (D) Colitis by TNBS increases the CINC-1 tissue expression and HBO decreases its expression. (E) Colitis by TNBS increases the IL-10 tissue expression and HBO decreases its expression. Data are expressed as the mean ± S.E.M. (n = 6–7). Data are expressed as the mean ± S.E.M. (n = 6–7). # P < 0.05 compared to the saline-injected group; *P < 0.05 compared to colitis (TNBS) group; (one-way ANOVA followed by the Bonferroni’s test).