Figure 6
From: Hyperbaric oxygen therapy ameliorates TNBS-induced acute distal colitis in rats
Hyperbaric oxygen reduces HIF-1α expression in acute distal colitis. Acute distal colitis was induced by intracolonic injection of TNBS (150 mg/kg). After two sessions of hyperbaric oxygen, the rats were killed, the distal 6 cm colonic segment was removed, and the expression of HIF-1α was determined by immunohistochemical analysis. (A) Group I (Saline), (B) Group II (Saline/HBO), (C) Group III (TNBS), (D) Group IV (TNBS/HBO), and (E) Rats with colitis (TNBS) presented substantial up-regulation of immunofluorescence of HIF-1α in the colon. The HBO treatment (TNBS/HBO) remarkably reduces HIF-1α expression. Data are expressed as the mean ± S.E.M. (n = 7). # P < 0.001 compared to the saline-injected group; *P < 0.001 compared to colitis (TNBS) group (one-way ANOVA followed by the Bonferroni’s test).